Uridine enhances the cytotoxic effect of D-glucosamine in rat C6 glioma cells.

  title={Uridine enhances the cytotoxic effect of D-glucosamine in rat C6 glioma cells.},
  author={Giuseppe Calvaruso and Gennaro Taibi and Maria Valeria Torregrossa and Nino Romano and Giovanni Tesoriere},
  journal={Life sciences},
  volume={39 23},
This paper studies the influence of uridine on the effects exerted by D-glucosamine in rat C6 glioma cells. 2 mM uridine increased markedly both the cytotoxic effect of the aminosugar and the inhibition of thymidine incorporation into acid-insoluble fraction. Furthermore the complete resumption of the capacity to incorporate either 3H-thymidine or 3H-mannose which was observed after the removal of the aminosugar, was impeded when the cells were treated contemporaneously with D-glucosamine and… 
The Tissue Concentration of UDP-N-acetylglucosamine Modulates the Stimulatory Effect of Insulin on Skeletal Muscle Glucose Uptake*
It is suggested that the marked reduction in insulin action induced by GlcN and uridine is mediated by increased accumulation of muscle UDP-N-acetylhexosamines, perhaps via altered glycosylation of protein(s) in GLUT4-containing vesicles.
Do antimetabolites interfere with the glycosylation of cellular glycoconjugates?
In the last decade the evidence is compelling that the variations in oligosaccharide structures observed coincide with changes in activities of specific glycosyltransferases, indicating the importance of these enzymes in the regulation of the Glycosylation process.
Dynamic O-linked glycosylation of nuclear and cytoskeletal proteins.
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    Annual review of biochemistry
  • 1997
Evidence is mounting that O-GlcNAcylation is an important regulatory modification that may have a reciprocal relationship with O-phosphorylation and may modulate many biological processes in eukaryotes.
Glucosamine and osteoarthritis
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The effect of D-glucosamine on the adenine and uridine nucleotides of sarcoma 180 ascites tumor cells.
Investigation of the effect of d-glucosamine on the metabolism of adenine and uridine nucleotides in Sarcoma 180 ascites tumor cells found no decrease in the intracellular ATP level was observed in ascites cells exposed to glucosamine in the presence of 10 mm glucose.
The inhibition of thymidine metabolism in tumor cells treated with D-glucosamine.
The amino sugar, D-glucosamine, inhibits the preformed route of thymidine metabolism in rat C6 glioma cells, and its effects, together with its known ability to reduce uridine nucleotide pools, may contribute to its toxicity toward certain experimental animal tumors.
Inhibitory effect of d-glucosamine and other sugar analogs on the viability and transplantability of ascites tumor cells.
d-Glucosamine has been shown to have a powerful cytotoxic effect on various ascites tumor lines, resulting in a decrease in viability and transplantability of the neoplastic cells, and d-Mannose was the only neutral sugar showing a cytot toxic effect on ascite tumor cells.
Inhibitory effects of D-glucosamine on the growth of Walker 256 carcinosarcoma and on protein, RNA, and DNA synthesis.
The resistance of rats and mice successfully treated with glucosamine to a second syngeneic tumor graft suggests that glucosamines treatment may interfere with the tumor in such a way as to permit the host to respond successfully to the antigenic makeup of the tumor.
D-glucosamine-induced changes in nucleotide metabolism and growth of colon-carcinoma cells in culture.
The growth-inhibitory effect of the amino sugar could therefore be best correlated with the quantitative change in the pattern of sugar nucleotides, and, in particular, with the many-fold increase in UDP-N-acetylglucosamine and UDP- nacetylgalactosamine.
Inhibitory effect of D-glucosamine and other sugars on the biosynthesis of protein, ribonucleic acid, and deoxyribonucleic acid in normal and neoplastic tissues.
The effect of exogenous glucosamine in vitro on the incorporation of the labeled precursors into normal tissues was much less than that into neoplastic tissues.
The inhibition of thymidine kinase in glial tumor cells by an amino sugar, D-glucosamine.
Thymidine kinase activity in rat C6 glioma cells is inhibited by 50 to 70% after 4 hr incubation with 20 mM D-glucosamine, and the kinetics of recovery of amino acid incorporation from inhibition was rapid, but the activity was depressed for at least 6 hr after drug washout.
The biochemical and ultrastructural effects of tunicamycin and D‐glucosamine in L1210 leukemic cells
Exposure of L1210 cells to tunicamycin resulted in specific alterations in the biochemical composition of the plasma membrane and in the inhibition of cellular agglutination by wheat germ agglutanin which were not apparent following exposure to equitoxic concentrations of the aminosugar.
Effects of D-glucosamine, D-mannosamine, and 2-deoxy-D-glucose on the ultrastructure of ascites tumor cells in vitro.
Ehrlich ascites carcinoma and Sarcoma 180 ascites tumor cells were treated in vitro with amino sugars, and the resulting changes were studied by light and electron microscopy. Addition of
Cytotoxic effects of D-glucosamine on the ultrastructures of normal and neoplastic tissues in vivo.
The most significant finding was the complete necrosis of tumor cells in rats sacrificed 5 days after 40 hr of infusion with D-glucosamine, accompanied by a remarkable recovery of the fine structure of the renal and hepatic parenchymal cells.