Uridine Diphosphate Glucose: the Coenzyme of the Galactose–Glucose Phosphate Isomerization

  title={Uridine Diphosphate Glucose: the Coenzyme of the Galactose–Glucose Phosphate Isomerization},
  author={Carlos E. Cardini and Alejandro C. Paladini and Ranwel Caputto and Luis F. Leloir},
A PREVIOUS report1 dealt with the enzymatic conversion of galactose-1-phosphate into glucose-1-phosphate and mentioned that a thermostable factor is necessary for the reaction. This factor has now been obtained practically pure, and for reasons given below will be referred to as uridine diphosphate glucose. 
The enzymatic transformation of uridine diphosphate glucose into a galactose derivative.
  • L. Leloir
  • Biology, Medicine
  • Archives of biochemistry and biophysics
  • 1951
It is concluded that the compound found with treatment of UDPG with an enzyme of S. fragilis is uridine diphosphate galactose, and the bearing of this finding on the mechanism of action of UDPG is discussed. Expand
The biosynthesis of glucosamine.
A partially purified enzyme has been prepared from Neurospora crassa which catalyzes the formation of glucosamine phosphate from hexose-6-phosphate and glutamine, which was identified by colour reactions, by dephosphorylation and paper chromatography and by its behaviour towards an acetylating system. Expand
Enzymic phosphorylation of galactosamine and galactose.
Evidence is presented indicating that the reaction product is galactosamine 1-phosphate, which was found to be more resistant to acid hydrolysis than aldose 1- phosphates. Expand
Non-enzymatic synthesis of the coenzymes, uridine diphosphate glucose and cytidine diphosphate choline, and other phosphorylated metabolic intermediates
The results suggest that metabolic intermediates could have been synthesized on the primitive Earth from simple precursors by means of prebiotic condensing agents. Expand
Guanosine diphosphate D-glucose glucohydrolase.
The possible significance of guanosine diphosphate glucose glucohydrolase and of sugar nucleotide phosphorylase in the control of the intracellular concentration of Guanosinediphosphates glucose and guanosinesine dphosphate mannose is discussed. Expand
Glucose activation and metabolism through UDP-glucose pyrophosphorylase in plants
The chiral stationary phase for UDP-glucose pyrophosphorylase has shown good chiral recognition ability and the ability to cleaveopolysaccharides and polypeptide chains is reported. Expand
A very simple synthesis of GlcNAc-alpha-pyrophosphoryl-decanol: a substrate for the assay of a bacterial galactosyltransferase.
A simple 'two-pot' synthesis is developed which is demonstrated here for GlcNAc-alpha-pyrophosphoryl-decanol and is shown to be an efficient acceptor for a bacterial galactosyltransferase. Expand
The biosynthesis of uridine diphosphate glucose and sucrose in sugar beet leaf.
Data show that sucrose is biosynthesized in the sugar-beet leaf as the product of a sequence of reactions, in which UDPG, formed from G-1-P and UTP, reacts with F-6-P to give sucrose phosphate. Expand
Aldo (and Keto) Hexoses and Uronic Acids
As soon as information became available at the beginning of this century concerning the structure of the different monosaccharide moieties of complex plant polysaccharides, hypotheses were advancedExpand
The Leloir pathway: a mechanistic imperative for three enzymes to change the stereochemical configuration of a single carbon in galactose
  • P. Frey
  • Chemistry, Medicine
  • FASEB journal : official publication of the Federation of American Societies for Experimental Biology
  • 1996
The structure of the enzyme reveals many details of the binding of NAD+ and inhibitors at the active site, and appears to have been guided by the principle of economy in the evolution of binding sites. Expand


The ultraviolet absorption spectra of the pyrimidine ribonucleosides and ribonucleotides.
Measurement of the absorption characteristics of the aminoand oxypurines in comparison with the free pyrimidines, cytosine and uracil and the corresponding ribonucleosides, cytidine and uridine. Expand
Le dosage photométrique des nucléosides puriques et pyrimidiques au moyen de la réaction a l'orcine
Resume Description d'une methode permettant de doser les nuclesides pyrimiques au moyen de la reation de Bial , modifiee d'apres Barrenscheeen et Peham , la methode permettant egalement de determinerExpand
The enzymatic transformation of galactose into glucose derivatives.