• DOI:10.1038/srep20136
• Corpus ID: 1643401

Uricase alkaline enzymosomes with enhanced stabilities and anti-hyperuricemia effects induced by favorable microenvironmental changes

@article{Zhou2016UricaseAE,
  title={Uricase alkaline enzymosomes with enhanced stabilities and anti-hyperuricemia effects induced by favorable microenvironmental changes},
  author={Yunli Zhou and Mi Zhang and Dan He and Xueyuan Hu and Hua-rong Xiong and Jianyong Wu and Biyue Zhu and Jingqing Zhang},
  journal={Scientific Reports},
  year={2016},
  volume={7}
}

• Yunli Zhou, Mi Zhang, +5 authors Jingqing Zhang
• Published 29 January 2016
• Biology
• Scientific Reports

Enzyme therapy is an effective strategy to treat diseases. Three strategies were pursued to provide the favorable microenvironments for uricase (UCU) to eventually improve its features: using the right type of buffer to constitute the liquid media where catalyze reactions take place; entrapping UCU inside the selectively permeable lipid vesicle membranes; and entrapping catalase together with UCU inside the membranes. The nanosized alkaline enzymosomes containing UCU/(UCU and catalase) (ESU/ESUC) in bicine buffer had better thermal, hypothermal, acid-base and proteolytic stabilities, in vitro and in vivo kinetic characteristics, and uric acid lowering effects. The favorable microenvironments were conducive to the establishment of the enzymosomes with superior properties. It was the first time that two therapeutic enzymes were simultaneously entrapped into one enzymosome having the right type of buffer to achieve added treatment efficacy. The development of ESU/ESUC in bicine buffer provides valuable tactics in hypouricemic therapy and enzymosomal application.