Uptake of metaraminol by the adrenal medullary granules.

@article{Lundborg1966UptakeOM,
  title={Uptake of metaraminol by the adrenal medullary granules.},
  author={P. Lundborg},
  journal={Acta physiologica Scandinavica},
  year={1966},
  volume={67 3},
  pages={
          423-9
        }
}
  • P. Lundborg
  • Published 1966
  • Chemistry, Medicine
  • Acta physiologica Scandinavica
Metaraminol is taken up by the adrenal medullary granules in vitro by displacing the endogenous catecholamines from their storage sites. This uptake is not blocked by reserpine. Metaraminol inhibits the ATP-Mg++ -dependent uptake of adrenaline but does not utilize this mechanism for uptake. There thus seem to exist two uptake mechanisms in the amine granules, i.e. one ATP- Mg++-dependent mechanism sensitive to reserpine, and the other acting by displacement of endogenous amines. Dopamine is… Expand
37 Citations
Inhibition of epinephrine and metaraminol uptake into adrenal medullary vesicles by aralkylamines and alkylamines
Abstract Two amine uptake mechanisms appeared to operate in isolated adrenal medullary storage vesicles; one site had a high affinity for epinephrine (Km ∼- 30 ωM) and low capacity (Umax ∼- 20Expand
Hypothetical model of catecholamine uptake into adrenal medullary storage vesicles
TLDR
A transport model is proposed in which catecholamines attach to the carrier on the outside of the membrane and are detached on the inside surface by the action of ATP; probable loci of action of uptake inhibitors (reserpine and N-ethylmaleimide) are identified. Expand
Mechanisms of accumulation of tyramine, metaraminol, and isoproterenol in isolated chromaffin granules and ghosts.
TLDR
The results indicate that serotonin and isoproterenol accumulated in isolated chromaffin granules and ghosts via a reserpine-sensitive mechanism, driven by the magnitude of the electrochemical proton gradient. Expand
DISCRIMINATION OF MONOAMINE UPTAKE BY MEMBRANES OF ADRENAL CHROMAFFIN GRANULES
TLDR
It is concluded that the membrane of adrenal chromaffin granules discriminates between the various monoamines with regard to the magnitude of their uptake and that two mechanisms of ATP‐stimulated uptake, one responsive and the other resistant to reserpine, exist at the level of this membrane. Expand
Fundamental Mechanisms in the Release of Catecholamines
In some endocrine tissues, such as the steroid-secreting glands, the release of the hormone is tightly coupled to its rate of biosynthesis because the tissue does not contain a significant store ofExpand
Stereospecificity and intracellular binding of metaraminol.
TLDR
The results suggest that d-MA is not firmly bound to the particulate fraction and is rapidly lost from sympathetically innervated tissues and it is further suggested that there is a transfer of MA from a more to a less available granular storage site with time. Expand
Morphine-reserpine interactions in the adrenal medulla.
TLDR
Chronic morphine enhances the adrenal CA-depleting effect of reserpine by a combination of increased reflex secretion, accelerated CA and vesicle turnover, and blockade of the CA-uptake system; prolonged reversal by Reserpine of some of the effects of chronic morphine may reflect a common site of action in the vesicles membrane. Expand
Fluorescent Histochemical Studies on the Uptake, Storage, and Release of Catecholamines
The fluorescence histochemical method of Falck and Hillarp has been used for studies on the adrenergic nerves, mainly in the rat. Some earlier findings are summarized as a background to theExpand
H+-Translocating ATPase and Other Membrane Enzymes Involved in the Accumulation and Storage of Biological Amines in Chromaffin Granules
Within the adrenal medulla, catecholamines accumulate into and are stored within a highly specialized subcellular organelle, the chromaffin granule. Since their isolation almost 30 years ago, anExpand
Steric aspects of adrenergic drugs.
TLDR
In vasa deferentia obtained from reserpine-pretreated rats, tyramine released equal amounts of radioactivity when tissues were either preincubated with (−)- or (+)-isomer, however, the response of vasadeferentia preincUBated with the (−)-isomers was approximately 7-fold greater than of those preincubsated withThe results are discussed in relation to the drug-induced release of the isomers. Expand
...
1
2
3
4
...

References

SHOWING 1-10 OF 13 REFERENCES
EFFECTS OF SOME PHARMACOLOGICALLY ACTIVE AMINES ON THE UPTAKE OF ARYLALKYLAMINES BY ADRENAL MEDULLARY GRANULES.
TLDR
Both reserpine and chlorpromazine are found to be competitive inhibitors of the adrenaline uptake by the granules, which means they compete with adrenaline for the same groups in the uptake mechanism for catecholamines. Expand
Uptake of Catecholamines by a Particulate Fraction of the Adrenal Medulla
Chromaffin granules isolated from beef adrenal medulla took up C14-labeled catecholamines from an isotonic medium at 37�C at a rate approximately 20 times the rate at 0�C. The uptake was stimulatedExpand
EFFECT OF ADENINE NUCLEOTIDES ON CATECHOLAMINE RELEASE AND UPTAKE IN ISOLATED ADRENERGIC NERVE GRANULES.
TLDR
On addition of 1–5 mM adenosine triphosphate (ATP) the spontaneous loss of noradrenaline from a suspension of adrenergic nerve granules is retarded or prevented and ADP and ATP enhance the uptake of catecholamines from the incubation fluid. Expand
The mechanism of catecholamine release by tyramine
Summary The incubation of isolated medullary granules from cattle causes a spontaneous release of catecholamines along with equivalent amounts of ATP. The sympathomimetic amines : tyramine,Expand
BINDING AND RELEASE OF METARAMINOL: MECHANISM OF NOREPINEPHRINE DEPLETION BY ALPHA-METHYL-M-TYROSINE AND RELATED AGENTS.
TLDR
It is concluded that l-metaraminol is the active norepinephrine-depleting agent following injection of α-MMT and that metaramineol exchanges with nore Pinephrine at adrenergic nerve endings on a mole-for-mole basis. Expand
A method for the fluorimetric determination of adrenaline and noradrenaline in tissues.
TLDR
A method for chemical assay of small amounts of adrenaline and noradrenaline in tissues is described, utilizing the difference in the activation spectra of the fluoro-phores to identify the two amines. Expand
Isolation and some biochemical properties of the catechol amine granules in the cow adrenal medulla.
  • N. Hillarp
  • Chemistry, Medicine
  • Acta physiologica Scandinavica
  • 1958
TLDR
The catechol amine granules in the cow adrenal medulla have been isolated free or practically free from mitochondria and microsomal material and show a high ATPase activity and probably contain an adenylate kinase. Expand
SOLUBILITY OF ACETYLENE IN LUNG TISSUE AS AN ERROR IN CARDIAC OUTPUT DETERMINATION WITH THE ACETYLENE METHOD.
The solubility of acetylene in lung tissue is measured by a rebreathing technique. Enough acetylene is dissolved in the lung tissue to simulate an extra gaseous lung volume between 0.274–0.48 liters.Expand
The Determination of Enzyme Dissociation Constants
On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, (ES), a t all values of the concentration of theExpand
MECHANISM OF AMINE TRANSPORT IN THE CELL MEMBRANES OF THE ADRENERGIC NERVES.
...
1
2
...