Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells of the rat liver: new insights into the classical vital staining and the reticulo-endothelial system

  title={Uptake of lithium carmine by sinusoidal endothelial and Kupffer cells of the rat liver: new insights into the classical vital staining and the reticulo-endothelial system},
  author={Y Kawai and B{\aa}rd Smedsr{\o}d and Kjetil H Elvevold and Kenjiro Wake},
  journal={Cell and Tissue Research},
Abstract Sinusoidal cells in the rat liver were studied in vivo and in vitro using the original vital staining with lithium carmine, which has contributed much to the development of the concept of the reticulo-endothelial system. Immunohistochemical and electron-microscopic studies revealed that the dye-incorporating cells were sinusoidal endothelial cells, Kupffer cells, and monocytes. The endothelial cells took up much more dye than did the Kupffer cells and bulged largely into the sinusoidal… 
Expression of a monocarboxylate transporter in reticular cells of the mouse lymph node and its involvement in the uptake of exogenous particles.
The selective localization of MCT1 and LYVE-1 suggests a high level of activity for lymphoid reticular cells in the uptake of carboxylate-modified and hyaluronate waste substances circulating in the body.
Uptake ability of hepatic sinusoidal endothelial cells and enhancement by lipopolysaccharide.
It is suggested that the sinusoidal endothelial cells can ingest not only soluble substances but also larger particles than those expected, and their uptake ability is strengthened under inflammatory conditions.
The selective distribution of LYVE-1-expressing endothelial cells and reticular cells in the reticulo-endothelial system (RES).
Findings suggest that the LYVE-1-expressing cells might be involved in the uptake of hyaluronan and other waste products as well as foreign particles circulating in the blood and lymph while participating in the subsequent degradation in relay with adjacent macrophage populations.
Prominent Receptors of Liver Sinusoidal Endothelial Cells in Liver Homeostasis and Disease
Seven Toll-like receptors have been investigated in LSECs, which are involved in the recognition and clearance of pathogen-associated molecular pattern as well as damage associated molecular pattern (DAMP).
Liver sinusoidal endothelial cells represents an important blood clearance system in pigs
It is shown here for the first time that pig LSEC, similar to what has been found earlier in rat L SEC, represent an effective scavenger system for removal of macromolecular waste products from the circulation.
Scavenger properties of cultivated pig liver endothelial cells
Pig LSEC express the five following categories of high capacity endocytic receptors: scavenger-, mannose-, hyaluronan-, collagen-, and IgG Fc-receptors, which suggest that pig LSEC have the same clearance activity as earlier described in rat LSEC.
Clearance function of scavenger endothelial cells
  • B. Smedsrød
  • Biology, Environmental Science
    Comparative hepatology
  • 2004
Experiments in other vertebrates, in particular the most numerous class, namely bony fishes, have shown that the specificity and mode of uptake is remarkably similar among vertebrates of considerably different phylogenetic age.
Rapid and Efficient Clearance of Blood-borne Virus by Liver Sinusoidal Endothelium
It is shown that nearly all infused adenovirus is cleared by the liver sinusoidal endothelial cell (LSEC), and the clearance mechanism to the LSEC is located and identified as a key site of antiviral activity.
Phenotypical and morphological alterations to rat sinusoidal endothelial cells in arterialized livers after portal branch ligation.
Arsenialization of the liver induces a partial (but not complete) transition of SECs into capillary-type endothelial cells, suggesting that arterialization might be one of the factors which induce sinusoidal capillarization in the development of hepatocellular carcinoma.