Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells.

@article{Bannon2002UptakeOL,
  title={Uptake of lead and iron by divalent metal transporter 1 in yeast and mammalian cells.},
  author={Desmond I Bannon and Matthew E Portnoy and Luisa Olivi and Peter St. John Lees and Valeria C Culotta and Joseph P. Bressler},
  journal={Biochemical and biophysical research communications},
  year={2002},
  volume={295 4},
  pages={978-84}
}
Although the divalent metal transporter (DMT1) was suggested to transport a wide range of metals in Xenopus oocytes, recent studies in other models have provided contrasting results. Here, we provide direct evidence demonstrating that DMT1 expressed in yeast mutants defective for high affinity iron transport facilitates the transport of iron with an 'apparent K(m)' of approximately 1.2 microM, and transport of lead with an 'apparent K(m)' of approximately 1.8 microM. DMT1-dependent lead… CONTINUE READING