Uptake of iopanoic acid by isolated rat hepatocytes in primary culture.

  title={Uptake of iopanoic acid by isolated rat hepatocytes in primary culture.},
  author={J. Barnhart and B. Witt and W. Hardison and R. Berk},
  journal={The American journal of physiology},
  volume={244 6},
  • J. Barnhart, B. Witt, +1 author R. Berk
  • Published 1983
  • Chemistry, Medicine
  • The American journal of physiology
  • Uptake of iopanoic acid (IOP) was studied in 3-day primary cultures of rat hepatocytes isolated by the collagenase perfusion method. 125I activity of cells after incubation with 125I-IOP (1.0-100 microM) was used as a measure of uptake. At each IOP concentration uptake was linear for the first 45 s. The initial uptake velocity was directly proportional to IOP concentration and was nonsaturable up to 100 microM. The calculated uptake rate constant was 0.67 nmol . mg prot-1 . min-1 . microM-1… CONTINUE READING

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