Uptake of N‐acetyl‐D‐mannosamine: an essential intermediate in polysialic acid biosynthesis by Escherichia coli K92

@article{RevillaNun1999UptakeON,
  title={Uptake of N‐acetyl‐D‐mannosamine: an essential intermediate in polysialic acid biosynthesis by Escherichia coli K92},
  author={B. Revilla-Nu{\'i}n and {\'A}. Reglero and M. A. Ferrero and L. Rodr{\'i}guez-Aparicio},
  journal={FEBS Letters},
  year={1999},
  volume={449}
}
The N‐acetyl‐D‐mannosamine (ManNAc) transport system of Escherichia coli K92 was studied when this bacterium was grown in a chemically defined medium containing ManNAc as carbon source. Kinetic measurements were carried out in vivo at 37°C in 25 mM phosphate buffer, pH 7.5. Under these conditions, the uptake rate was linear for at least 15 min and the calculated K m for ManNAc was 280 μM. The transport system was strongly inhibited by sodium arsenate (97%), potassium cyanide (84%) and 2,4… Expand
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