Uptake of FITC-Chitosan Nanoparticles by A549 Cells

  title={Uptake of FITC-Chitosan Nanoparticles by A549 Cells},
  author={Min Huang and Zengshuan Ma and E. W. Khor and L. R. Lim},
  journal={Pharmaceutical Research},
Purpose. The objective of this study was to evaluate the extent and mechanism of uptake of fluorescent chitosan nanoparticles by the A549 cells, a human cell line derived from the respiratory epithelium. Methods. Covalent conjugation with fluorescein-5-isothiocyanate yielded stably labeled chitosan molecules, which were successfully formulated into nanoparticles by ionotropic gelation. Uptake of fluorescein-5-isothiocyanate-chitosan nanoparticles and chitosan molecules by confluent A549 cells… CONTINUE READING