Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene.

@article{VzquezBermdez2000UptakeO2,
  title={Uptake of 2-oxoglutarate in Synechococcus strains transformed with the Escherichia coli kgtP gene.},
  author={Mar{\'i}a F{\'e}lix V{\'a}zquez-Berm{\'u}dez and Ana Herrero and Enrique Flores},
  journal={Journal of bacteriology},
  year={2000},
  volume={182 1},
  pages={211-5}
}
A number of cyanobacteria from different taxonomic groups exhibited very low levels of uptake of 2-[U-(14)C]oxoglutarate. Synechococcus sp. strain PCC 7942 was transformed with DNA constructs carrying the Escherichia coli kgtP gene encoding a 2-oxoglutarate permease and a kanamycin resistance gene cassette. The Synechococcus sp. strains bearing the kgtP gene incorporated 2-oxoglutarate into the cells through an active transport process. About 75% of the radioactivity from the 2-[U-(14)C… CONTINUE READING