Uptake, release and novel species-dependent oxygenation of arachidonic acid in human and animal airway epithelial cells.

@article{Holtzman1988UptakeRA,
  title={Uptake, release and novel species-dependent oxygenation of arachidonic acid in human and animal airway epithelial cells.},
  author={Michael J Holtzman and J R Hansbrough and Glenn Rosen and John Turk},
  journal={Biochimica et biophysica acta},
  year={1988},
  volume={963 3},
  pages={401-13}
}
To determine identities of mediators and mechanisms for their release from pulmonary airway epithelial cells, we examined the capacities of epithelial cells from human, dog and sheep airways to incorporate, release and oxygenate arachidonic acid. Purified cell suspensions were incubated with radiolabeled arachidonic acid and/or ionophore A23187; fatty acid esterification and hydrolysis were traced chromatographically, and oxygenated metabolites were identified using high-pressure liquid… CONTINUE READING