Upstream process optimization of polyhydroxybutyrate (PHB) by Alcaligenes latus using two-stage batch and fed-batch fermentation strategies.

@article{Wang2012UpstreamPO,
  title={Upstream process optimization of polyhydroxybutyrate (PHB) by Alcaligenes latus using two-stage batch and fed-batch fermentation strategies.},
  author={Bingqing Wang and Ratna R. Sharma-Shivappa and Jonathan W. Olson and Saad Akhtar Khan},
  journal={Bioprocess and biosystems engineering},
  year={2012},
  volume={35 9},
  pages={1591-602}
}
This research focused on optimizing the upstream process time for production of polyhydroxybutyrate (PHB) from sucrose by two-stage batch and fed-batch fermentation with Alcaligenes latus ATCC 29714. The study included selection of strain, two-stage batch fermentations with different time points for switching to nitrogen limited media (14, 16 or 18 h) and fed-batch fermentations with varied time points (similar to two stage) for introducing nitrogen limited media. The optimal strain to produce… CONTINUE READING