Up-regulation of opioid gene expression in spinal cord evoked by experimental nerve injuries and inflammation

  title={Up-regulation of opioid gene expression in spinal cord evoked by experimental nerve injuries and inflammation},
  author={Gaetano Draisci and Keith C. Kajander and Ronald Dubner and Gary J. Bennett and Michael J. Iadarola},
  journal={Brain Research},

Molecular Biology of Dynorphin Gene Expression in Relationship to Spinal Cord Processing of Pain

Peripheral inflammation and experimental neuropathic pain are known to induce a series of fundamental modifications of gene expression in neurons of the spinal cord dorsal horn that may modulate or underlie neuronal excitability changes established over time in the dorsal horn during inflammation.

Spinal Cord Neuronal Plasticity: Mechanisms of Persistent Pain Following Tissue Damage and Nerve Injury

Findings have important therapeutic implications because the pain of tissue and nerve damage can now be attacked at the site of injury where it is initiated and at central nervous system sites Where it is maintained.



Induction of c-fos-like protein in spinal cord neurons following sensory stimulation

Physiological stimulation of rat primary sensory neurons causes the expression of c-fos-protein-like immunoreactivity in nuclei of postsynaptic neurons of the dorsal horn of the spinal cord, suggesting that synaptic transmission may induce rapid changes in gene expression in certain post Synaptic neurons.

In situ hybridization histochemistry and immunocytochemistry reveal an increase in spinal dynorphin biosynthesis in a rat model of peripheral inflammation and hyperalgesia.

Since neurons demonstrating the increase in dynorphin biosynthesis are located in both the superficial and deep dorsal horn laminae, the data provide evidence for opioid modulation of nociceptive neural circuits in these two distinct spinal locations.

Expression of c‐fos protein in interneurons and projection neurons of the rat spinal cord in response to noxious somatic, articular, and visceral stimulation

The more deeply located cells, of the dorsal and medioventral horns, had the most extensive rostrocaudal spread; they were found from L1 through the rostral sacral segments.

Increased spinal cord dynorphin mRNA during peripheral inflammation.

The parallel elevations of the mRNA and peptide product suggest that an increased activity of dynorphin neurons occurs in spinal cord in response to altered afferent input due to the peripheral inflammatory process.