Up-promoter mutations in the trpBA operon of Pseudomonas aeruginosa.

@article{Han1991UppromoterMI,
  title={Up-promoter mutations in the trpBA operon of Pseudomonas aeruginosa.},
  author={Chang Yong Han and Irving P. Crawford and Caroline S Harwood},
  journal={Journal of bacteriology},
  year={1991},
  volume={173 12},
  pages={
          3756-62
        }
}
In Pseudomonas aeruginosa, the operon encoding tryptophan synthase (trpBA) is positively regulated by the TrpI protein and an intermediate in tryptophan biosynthesis, indoleglycerol phosphate (InGP). A gene fusion in which the trpBA promoter directs expression of the Pseudomonas putida xylE gene was constructed. By using a P. putida F1 todE mutant carrying this fusion on a plasmid, three cis-acting mutations that increased xylE expression enough to allow the todE strain to grow on toluene were… CONTINUE READING

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