Up-promoter mutations in the trpBA operon of Pseudomonas aeruginosa.

  title={Up-promoter mutations in the trpBA operon of Pseudomonas aeruginosa.},
  author={C. Y. Han and I. Crawford and C. Harwood},
  journal={Journal of bacteriology},
  volume={173 12},
  • C. Y. Han, I. Crawford, C. Harwood
  • Published 1991
  • Biology, Medicine
  • Journal of bacteriology
  • In Pseudomonas aeruginosa, the operon encoding tryptophan synthase (trpBA) is positively regulated by the TrpI protein and an intermediate in tryptophan biosynthesis, indoleglycerol phosphate (InGP). A gene fusion in which the trpBA promoter directs expression of the Pseudomonas putida xylE gene was constructed. By using a P. putida F1 todE mutant carrying this fusion on a plasmid, three cis-acting mutations that increased xylE expression enough to allow the todE strain to grow on toluene were… CONTINUE READING
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