Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site.

@article{Boer2006UnveilingTM,
  title={Unveiling the molecular mechanism of a conjugative relaxase: The structure of TrwC complexed with a 27-mer DNA comprising the recognition hairpin and the cleavage site.},
  author={Roeland Boer and Silvia Russi and Alicia Guasch and Mar{\'i}a Lucas and Alexandre G. Blanco and Rosa P{\'e}rez-Luque and Miquel Coll and F Sebasti{\'a}n de la Cruz},
  journal={Journal of molecular biology},
  year={2006},
  volume={358 3},
  pages={857-69}
}
TrwC is a DNA strand transferase that catalyzes the initial and final stages of conjugative DNA transfer. We have solved the crystal structure of the N-terminal relaxase domain of TrwC in complex with a 27 base-long DNA oligonucleotide that contains both the recognition hairpin and the scissile phosphate. In addition, a series of ternary structures of protein-DNA complexes with different divalent cations at the active site have been solved. Systematic anomalous difference analysis allowed us to… CONTINUE READING

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photorealistic molecular graphics

  • E. Merritt, Bacon, D. . Raster3D
  • In Methods in Enzymology (Carter, C., Jr & Sweet…
  • 2006
1 Excerpt

IS91 rolling-circle transposition

  • M. P. Garcillan-Barcia, I. Bernales, M. V. Mendiola, F. de la Cruz Fd
  • In Mobile DNA II (Craig,
  • 2005
1 Excerpt

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