Unveiling cells’ local environment during cryopreservation by correlative in situ spatial and thermal analyses

@article{Qin2020UnveilingCL,
  title={Unveiling cells’ local environment during cryopreservation by correlative in situ spatial and thermal analyses},
  author={Kankan Qin and Corentin Eschenbrenner and F. Ginot and D. Dedovets and T. Coradin and S. Deville and F. Fernandes},
  journal={bioRxiv},
  year={2020}
}
Cryopreservation is the only fully established procedure to extend the lifespan of living cells and tissues, a key to activities spanning from fundamental biology to clinical practice. Despite its prevalence and impact, central aspects of cryopreservation, such as the cell’s physico-chemical environment during freezing, remain elusive. Here we address that question by coupling in situ microscopic directional freezing to visualize cells and their surroundings during freezing with the freezing… Expand
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