Unveiling cells’ local environment during cryopreservation by correlative in situ spatial and thermal analyses

  title={Unveiling cells’ local environment during cryopreservation by correlative in situ spatial and thermal analyses},
  author={Kankan Qin and Corentin Eschenbrenner and F. Ginot and D. Dedovets and T. Coradin and S. Deville and F. Fernandes},
Cryopreservation is the only fully established procedure to extend the lifespan of living cells and tissues, a key to activities spanning from fundamental biology to clinical practice. Despite its prevalence and impact, central aspects of cryopreservation, such as the cell’s physico-chemical environment during freezing, remain elusive. Here we address that question by coupling in situ microscopic directional freezing to visualize cells and their surroundings during freezing with the freezing… Expand
Colonization versus encapsulation in cell-laden materials design: porosity and process biocompatibility determine cellularization pathways
A simple two-dimensional map based on materials' pore size and the cytocompatibility of their fabrication process is proposed to draw, for the first time, a guide to building cellularized materials. Expand


Advances in the slow freezing cryopreservation of microencapsulated cells
This review will discuss the different steps that should be understood and taken into account to preserve microencapsulated cells by slow freezing in a successful and simple manner and discuss some recommendations that the research community may pursue to optimize the preservation of microencapulated cells. Expand
Cryopreservation and its clinical applications
Continuous understanding of the physical and chemical properties that occur in the freezing and thawing cycle will be necessary for the successful cryopreservation of cells or tissues and their clinical applications. Expand
Polyampholytes as Emerging Macromolecular Cryoprotectants
This Perspective will introduce polyampholytes as emerging macromolecular cryoprotective agents and demonstrate they have the potential to impact a range of fields from cell-based therapies to basic cell biology and may be able to improve, or replace, current solvent-based cryoprotsective agents. Expand
Directional freezing for the cryopreservation of adherent mammalian cells on a substrate
A controlled slow cooling method combining initial directional freezing followed by gradual cooling down to -80°C for robust preservation of cell monolayers adherent to a substrate is proposed and can be readily applied for cryopreservation of cellular cultures in microfluidic devices. Expand
Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide.
The use of Me(2)SO at low concentrations in cell freezing solutions may support the development of clinical trials of AFSCs and develop non-toxic CPAs or reduce CPA concentration in the freezing media used. Expand
The Unusual Properties of Polytetrafluoroethylene Enable Massive-Volume Vitrification of Stem Cells with Low-Concentration Cryoprotectants.
Polytetrafluoroethylene (PTFE, best known as Teflon for making non-stick cookware) capillary is flexible and unusually stable at a cryogenic temperature and may greatly facilitate the use of stem cell-based constructs for tissue regeneration and cell based therapies in the clinic. Expand
Principles of cryopreservation by vitrification.
This chapter describes the basic principles of vitrification and indicates the broad potential biological relevance of vitrified systems. Expand
Apatite nanoparticles strongly improve red blood cell cryopreservation by mediating trehalose delivery via enhanced membrane permeation.
This approach is a new alternative to using toxic glycerol for cells cryopreservation, and the identification of this enhancing no-pore permeation mechanism of apatite NP appears as an original delivery pathway for cryoprotectant agents and beyond. Expand
Glycerol-Free Cryopreservation of Red Blood Cells Enabled by Ice-Recrystallization-Inhibiting Polymers.
The cryopreservation of erythrocytes with storage at -20 °C using hydroxyethyl starch (HES) and the ice recrystallization inhibitor poly(vinyl alcohol) (PVA), which is a biomimetic of naturally occurring antifreeze (glyco)proteins (AF(G)Ps), is described. Expand
Ice Recrystallization Inhibiting Polymers Enable Glycerol-Free Cryopreservation of Microorganisms
This work introduces a new concept for the storage/transport of microorganisms by using ice recrystallization inhibiting poly(vinyl alcohol) in tandem with poly(ethylene glycol), and represents a step-change in how microorganisms will be stored by the design of new macromolecular ice growth inhibitors. Expand