Unusual synthesis by the Escherichia coli

  title={Unusual synthesis by the Escherichia coli},
  author={Ming Xiao Hou},
The tRNA 39 end contains the conserved CCA sequence at the 74–76 positions. The CCA sequence is synthesized and maintained by the CCA-adding enzymes. The specificity of the Escherichia coli enzyme at each of the 74–76 positions was investigated using synthetic minihelix substrates that contain permuted 39 ends. Results here indicate that the enzyme has the ability to synthesize unusual 3 9 ends. When incubated with CTP alone, the enzyme catalyzed the addition of C74, C75, C76, and multiple Cs… CONTINUE READING