Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba.

@article{Pfeiffer1993UnusualSO,
  title={Unusual structure of the double-stranded RNA associated with the '447' cytoplasmic male sterility in Vicia faba.},
  author={Peter Pfeiffer and Jae Lark Jung and Jacques Heitzler and G{\'e}rard Keith},
  journal={The Journal of general virology},
  year={1993},
  volume={74 ( Pt 6)},
  pages={
          1167-73
        }
}
The 16.7 kbp dsRNA specific to the '447' cytoplasmic male sterility (CMS) line of Vicia faba was labelled in vitro with [alpha-32P]ATP and poly(A) polymerase, and by T4 RNA ligase-mediated addition of [32P]pCp. Analysis of the reaction products under denaturing conditions revealed in both cases extensive labelling of a 4.5 kb ssRNA, already detected in previous experiments in which the RNA-dependent RNA polymerase associated with the dsRNA was allowed to pursue RNA synthesis on preinitiated… CONTINUE READING

References

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On the mechanism of cytoplasmic male sterility in the 447 line of Vicia faba

  • T. TURVEN, S. J. GARGER, L. K. GRILL
  • Plant Molecular Biology
  • 1988

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