Unusual stability of the Methanospirillum hungatei sheath.

@article{Beveridge1985UnusualSO,
  title={Unusual stability of the Methanospirillum hungatei sheath.},
  author={Terry J. Beveridge and Murray Stewart and Ronald J. Doyle and G. Dennis Sprott},
  journal={Journal of bacteriology},
  year={1985},
  volume={162 2},
  pages={728-37}
}
The proteinaceous sheath of Methanospirillum hungatei was isolated by lysing cells in 50 mM dithiothreitol, separating the sheath from other cellular material by discontinuous sucrose density centrifugation, and removing the "cell spacers" with dilute NaOH. The isolated sheath material consisted of hollow tubes which had a highly ordered surface array. The stability of the sheath to treatment with denaturants and to enzymatic digestion was examined by a turbidimetric assay in conjunction with… CONTINUE READING
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