Unsuitability of lymphoblastoid cell lines as surrogate of cryopreserved isolated lymphocytes for the analysis of DNA double-strand break repair activity.

@article{Zijno2010UnsuitabilityOL,
  title={Unsuitability of lymphoblastoid cell lines as surrogate of cryopreserved isolated lymphocytes for the analysis of DNA double-strand break repair activity.},
  author={Andrea Zijno and Paola Porcedda and Francesca Saini and Alessandra Allione and Bruno Garofalo and Francesca Marcon and Simonetta Guarrera and Valentina Turinetto and Valentina Minieri and Ada Funaro and Riccardo Crebelli and Claudia Giachino and Giuseppe Matullo},
  journal={Mutation research},
  year={2010},
  volume={684 1-2},
  pages={98-105}
}
As first task of a comprehensive investigation on DNA repair genotype-phenotype correlations, the suitability of Epstein-Barr virus (EBV)-transformed lymphoblastoid cell lines (LCLs) as surrogate of cryopreserved peripheral blood mononuclear cells (PBMCs) in DNA repair phenotypic assays was evaluated. To this aim the amount of DNA damage induced by gamma-rays and DNA repair capacity were evaluated in unstimulated (G(0)) and mitogen-simulated (G(2)) PBMC from 20 healthy subjects and in EBV… CONTINUE READING

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To this aim the amount of DNA damage induced by gamma - rays and DNA repair capacity were evaluated in unstimulated ( G(0 ) ) and mitogen - simulated ( G(2 ) ) PBMC from 20 healthy subjects and in EBV - transformed LCL obtained from the same individuals .
To this aim the amount of DNA damage induced by gamma - rays and DNA repair capacity were evaluated in unstimulated ( G(0 ) ) and mitogen - simulated ( G(2 ) ) PBMC from 20 healthy subjects and in EBV - transformed LCL obtained from the same individuals .
DNA RepairBiological process results from biological processDNA Damage
To this aim the amount of DNA damage induced by gamma - rays and DNA repair capacity were evaluated in unstimulated ( G(0 ) ) and mitogen - simulated ( G(2 ) ) PBMC from 20 healthy subjects and in EBV - transformed LCL obtained from the same individuals .
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