Unstainable DNA in cell nuclei. Comparison of ten different fluorochromes.

@article{Darzynkiewicz1988UnstainableDI,
  title={Unstainable DNA in cell nuclei. Comparison of ten different fluorochromes.},
  author={Zbigniew Darzynkiewicz and Frank Traganos},
  journal={Analytical and quantitative cytology and histology},
  year={1988},
  volume={10 6},
  pages={462-6}
}
Ten fluorochromes with specificity for DNA were used to compare the stainability of nuclei of exponentially growing, nondifferentiated Friend leukemia (FL) cells with that of dimethylsulfoxide-induced, fully differentiated FL cell nuclei. Decreased accessibility of DNA to several dyes, particularly pronounced in the case of some intercalators, was observed… CONTINUE READING