Unstable expression and amplification of a transfected oncogene in confluent and subconfluent cells.

@article{Glanville1985UnstableEA,
  title={Unstable expression and amplification of a transfected oncogene in confluent and subconfluent cells.},
  author={N. Theresa Glanville},
  journal={Molecular and cellular biology},
  year={1985},
  volume={5 6},
  pages={
          1456-64
        }
}
NIH 3T3 cells were transfected with a plasmid containing the transforming gene, v-src, from Rous sarcoma virus. One of the transformed cell lines isolated reverted to a flat, nontransformed morphology after cloning through soft agar. This cell line did not express the src gene and could no longer grow in soft agar. When these cells were held at confluence, spontaneous foci appeared which eventually covered the dish. The appearance of foci correlated with an increase in v-src gene expression… CONTINUE READING
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