Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase.

@article{GattiLafranconi2008UnscramblingTS,
  title={Unscrambling thermal stability and temperature adaptation in evolved variants of a cold-active lipase.},
  author={Pietro Gatti-Lafranconi and Serena M Caldarazzo and Alessandra Villa and Lilia Alberghina and Marina Lotti},
  journal={FEBS letters},
  year={2008},
  volume={582 15},
  pages={2313-8}
}
Directed evolution by error-prone PCR was applied to stabilize the cold-active lipase from Pseudomonas fragi (PFL). PFL displays high activity at 10 degrees C, but it is highly unstable even at moderate temperatures. After two rounds of evolution, a variant was generated with a 5-fold increase in half-life at 42 degrees C and a shift of 10 degrees C in the temperature optimum, nevertheless retaining cold-activity. The evolved lipase displayed specific activity higher than the wild type enzyme… CONTINUE READING