Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines

  title={Unraveling multi-state molecular dynamics in single-molecule FRET experiments. I. Theory of FRET-lines},
  author={Anders Barth and O. A. Opanasyuk and Thomas O. Peulen and Suren Felekyan and Stanislav Kalinin and Hugo Sanabria and Claus A. M. Seidel},
  journal={The Journal of Chemical Physics},
Conformational dynamics of biomolecules are of fundamental importance for their function. Single-molecule studies of Förster Resonance Energy Transfer (smFRET) between a tethered donor and acceptor dye pair are a powerful tool to investigate the structure and dynamics of labeled molecules. However, capturing and quantifying conformational dynamics in intensity-based smFRET experiments remains challenging when the dynamics occur on the sub-millisecond timescale. The method of multiparameter… 

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