Unraveling V(D)J Recombination Insights into Gene Regulation

@article{Jung2004UnravelingVR,
  title={Unraveling V(D)J Recombination Insights into Gene Regulation},
  author={David Jung and Frederick W. Alt},
  journal={Cell},
  year={2004},
  volume={116},
  pages={299-311}
}
V(D)J recombination assembles antigen receptor genes from component gene segments. We review findings that have shaped our current understanding of this remarkable mechanism, with a focus on two major reports--the first detailed comparison of germline and rearranged antigen receptor loci and the discovery of the recombination activating gene-1. 
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This chapter summarizes the current knowledge and highlights unanswered questions regarding the regulation of V(D)J recombination at the Tcrb locus, placing emphasis on mouse transgenesis and gene-targeting approaches. Expand
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TLDR
Current knowledge regarding the regulation of V(D)J recombination at the Tcrb gene locus is summarized. Expand
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TLDR
Advances that have been made in understanding how V(D)J recombination at the IgH locus is controlled are summarized and important areas for future investigation are discussed. Expand
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TLDR
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RAG Chromatin Scanning During V(D)J Recombination and Chromatin Loop Extrusion are Related Processes.
TLDR
This review covers recent work that has elucidated RAG structure and work that revealed that RAG has a novel chromatin scanning activity that contributes to its ability to locate V, D, J gene segment substrates within large chromosomal loop domains bounded by CTCF-binding elements (CBEs). Expand
Role of non-homologous end joining in V(D)J recombination
TLDR
The efforts of a number of laboratories are summarized in providing the mechanisms of V(D)J regulation with a focus on the NHEJ pathway, which allows efficient and regulated generation of the antigen receptors. Expand
Turning T‐cell receptor β recombination on and off: more questions than answers
TLDR
Evaluated current understanding of developmental regulation at the Tcrb locus highlights the many unresolved issues and discusses how recent concepts emerging from studies of other antigen receptor loci may (or may not) help to resolve these issues. Expand
Reassignment of the murine 3′TRDD1 recombination signal sequence
TLDR
The variability introduced in signal joints generated after rearrangement of the TR DD1 and TRDD2 genes in murine thymocytes is analyzed and it is shown that the functional TRDD1 recombination signal is shifted inside the putative coding sequence of theTRDD1 gene and, consequently, that this gene is shorter than indicated in the databases. Expand
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