Unoccupied binding sites for oestrogen in nuclei of a breast tumour cell line (MCF-7).

@article{Geier1981UnoccupiedBS,
  title={Unoccupied binding sites for oestrogen in nuclei of a breast tumour cell line (MCF-7).},
  author={Andrea Geier and Michal Haimsohn and Zvi Malik and Bruno Lunenfeld},
  journal={The Biochemical journal},
  year={1981},
  volume={200 3},
  pages={515-20}
}
1. A method to measure both occupied and unoccupied oestrogen receptors directly in the crude nuclear fraction of the MCF-7 cells was developed. The receptors had high affinity for oestradiol (Kd approx. 0.7 nM) and binding specificity characteristics of oestrogen receptors. 2. A substantial amount of the unoccupied receptors were found in the crude nuclear fraction. 3. Several experiments excluded the possibility that the unoccupied nuclear receptor might be a cytoplasmic contaminant. (a… CONTINUE READING