Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma

@inproceedings{Hirao2012UnnaturalBP,
  title={Unnatural base pair systems toward the expansion of the genetic alphabet in the central dogma},
  author={Ichiro Hirao and Michiko Kimoto},
  booktitle={Proceedings of the Japan Academy. Series B, Physical and biological sciences},
  year={2012}
}
Toward the expansion of the genetic alphabet of DNA, several artificial third base pairs (unnatural base pairs) have been created. Synthetic DNAs containing the unnatural base pairs can be amplified faithfully by PCR, along with the natural A-T and G-C pairs, and transcribed into RNA. The unnatural base pair systems now have high potential to open the door to next generation biotechnology. The creation of unnatural base pairs is a consequence of repeating "proof of concept" experiments. In the… CONTINUE READING