Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment.

@article{Huang2008UnnaturalAA,
  title={Unnatural amino acid replacement in a yeast G protein-coupled receptor in its native environment.},
  author={Li-Yin Huang and George K.E. Umanah and Melinda Hauser and Cagdas Devrim Son and Boris Arshava and Fred R. Naider and Jeffrey M. Becker},
  journal={Biochemistry},
  year={2008},
  volume={47 20},
  pages={5638-48}
}
Ste2p is the G protein-coupled receptor (GPCR) for the tridecapeptide pheromone alpha factor of Saccharomyces cerevisiae. This receptor-pheromone pair has been used extensively as a paradigm for investigating GPCR structure and function. Expression in yeast harboring a cognate tRNA/aminoacyl-tRNA synthetase pair specifically evolved to incorporate p-benzoyl- l-phenylalanine (Bpa) in response to the amber codon allowed the biosynthesis of Bpa-substituted Ste2p in its native cell. We replaced… CONTINUE READING

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