Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.

@article{Seipp2007UnlabeledOA,
  title={Unlabeled oligonucleotides as internal temperature controls for genotyping by amplicon melting.},
  author={Michael T Seipp and Jacob Durtschi and Michael Liew and Jamie Williams and Kristy Damjanovich and Genevi{\`e}ve A. Pont-Kingdon and Elaine Lyon and Karl V. Voelkerding and Carl T. Wittwer},
  journal={The Journal of molecular diagnostics : JMD},
  year={2007},
  volume={9 3},
  pages={284-9}
}
Amplicon melting is a closed-tube method for genotyping that does not require probes, real-time analysis, or allele-specific polymerase chain reaction. However, correct differentiation of homozygous mutant and wild-type samples by melting temperature (Tm) requires high-resolution melting and closely controlled reaction conditions. When three different DNA extraction methods were used to isolate DNA from whole blood, amplicon Tm differences of 0.03 to 0.39 degrees C attributable to the… CONTINUE READING

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