Universal sample preparation method for proteome analysis

  title={Universal sample preparation method for proteome analysis},
  author={J. Wisniewski and Alexandre Zougman and Nagarjuna Nagaraj and M. Mann},
  journal={Nature Methods},
  • J. Wisniewski, Alexandre Zougman, +1 author M. Mann
  • Published 2009
  • Chemistry, Medicine
  • Nature Methods
  • We describe a method, filter-aided sample preparation (FASP), which combines the advantages of in-gel and in-solution digestion for mass spectrometry–based proteomics. [...] Key Result We completely solubilized the proteome in sodium dodecyl sulfate, which we then exchanged by urea on a standard filtration device. Peptides eluted after digestion on the filter were pure, allowing single-run analyses of organelles and an unprecedented depth of proteome coverage.Expand Abstract
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