Universal reference method for real-time PCR gene expression analysis of preimplantation embryos.

@article{Bower2007UniversalRM,
  title={Universal reference method for real-time PCR gene expression analysis of preimplantation embryos.},
  author={Neil Ivan Bower and Ralf Joachim Moser and Jonathan Robert Hill and Sigrid A. Lehnert},
  journal={BioTechniques},
  year={2007},
  volume={42 2},
  pages={
          199-206
        }
}
Real-time PCR expression profiling in individual preimplantation embryos poses two main challenges. First, the amount of RNA from blastocysts (between 100 and 200 cells) is too small to quantify, and secondly, a reference gene with stable expression across preimplantation embryos produced by different reproductive technologies is required. We have developed a method using RNA and DNA spikes that allows for accurate normalization of gene expression without the use of an internal housekeeping… CONTINUE READING