Universal primer set for the full-length amplification of all influenza A viruses

@article{Hoffmann2001UniversalPS,
  title={Universal primer set for the full-length amplification of all influenza A viruses},
  author={Erich Hoffmann and J{\"u}rgen Stech and Yi Guan and Robert James Webster and Daniel Roberto Perez},
  journal={Archives of Virology},
  year={2001},
  volume={146},
  pages={2275-2289}
}
 To systematically identify and analyze the 15 HA and 9 NA subtypes of influenza A virus, we need reliable, simple methods that not only characterize partial sequences but analyze the entire influenza A genome. We designed primers based on the fact that the 15 and 21 terminal segment specific nucleotides of the genomic viral RNA are conserved between all influenza A viruses and unique for each segment. The primers designed for each segment contain influenza virus specific nucleotides at their 3… CONTINUE READING
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