Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction.

@article{Adzhar1996UniversalOF,
  title={Universal oligonucleotides for the detection of infectious bronchitis virus by the polymerase chain reaction.},
  author={A B Adzhar and Karen Shaw and Paul Britton and David Cavanagh},
  journal={Avian pathology : journal of the W.V.P.A},
  year={1996},
  volume={25 4},
  pages={817-36}
}
The universality of seven pairs of oligonucleotides for detection of the coronavirus infectious bronchitis virus (IBV) by reverse-transcription polymerase chain reaction (RT-PCR) was examined using 41 isolates of IBV collected over five decades from Europe, Japan and the USA. Oligonucleotides specific for sequences within the S2 region of the spike (S) gene (Lin et al., 1991a) and nucleocapsid (N) gene (Zwaagstra et al., 1992) gave the appropriate products with all 41 isolates. Oligonu-cleotide… CONTINUE READING

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