Universal fungus-specific primer systems and group-specific hybridization oligonucleotides for 18S rDNA.

@article{Kappe1996UniversalFP,
  title={Universal fungus-specific primer systems and group-specific hybridization oligonucleotides for 18S rDNA.},
  author={Reinhard Kappe and Claudius Fauser and Charles N Okeke and Matthias Maiwald},
  journal={Mycoses},
  year={1996},
  volume={39 1-2},
  pages={25-30}
}
We designed two primer systems that amplify a fragment of the gene coding for the small ribosomal subunit (18S rRNA). A broadly reactive, yet fungus-specific, primer cocktail comprises two previously published primers, TR1 and TR2, which specifically amplify dermatophytes, and two newly designed primers, CA1 and AF2, which specifically amplify Candida and Aspergillus respectively. This primer cocktail amplifies a DNA fragment of approximately 578 basepairs (bp) in length (from position 838 to… CONTINUE READING

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