Universal fluorescent labeling (UFL) method for automated microsatellite analysis.

  title={Universal fluorescent labeling (UFL) method for automated microsatellite analysis.},
  author={Masashi Shimizu and Natsuki Kosaka and Takashi Shimada and Takemitsu Nagahata and Hironori Iwasaki and Hisaki Nagai and Tadayoshi Shiba and Mitsuru Emi},
  journal={DNA research : an international journal for rapid publication of reports on genes and genomes},
  volume={9 5},
We have devised a novel method for automated microsatellite analysis using "universal" fluorescent labeling. This system is based on polymerase chain reactions driven by sequence-specific primers and a reporter primer labeled with a fluorescent dye at its 5' end. The forward sequence-specific primer is designed with a tag region bearing no homology to any human genomic sequence. Complementary tag sequences act as templates for the 6-carboxyfluorescein-labeled reporter primer, and those products… CONTINUE READING
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