Unique self-palmitoylation activity of the transport protein particle component Bet3: a mechanism required for protein stability.

@article{Kmmel2006UniqueSA,
  title={Unique self-palmitoylation activity of the transport protein particle component Bet3: a mechanism required for protein stability.},
  author={Daniel K{\"u}mmel and Udo Heinemann and Michael Veit},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2006},
  volume={103 34},
  pages={12701-6}
}
Bet3 is a component of the transport protein particle complex involved in vesicular trafficking to and through the Golgi complex. X-ray structural analysis of human and mouse Bet3 revealed a hydrophobic tunnel inside the protein, which is occupied by a fatty acid linked to cysteine-68. We show here that Bet3 has strong self-palmitoylating activity. Incubation of purified Bet3 with [3H]palmitoyl-CoA (Pal-CoA) leads to a rapid and stoichiometric attachment of fatty acids to cysteine-68. Bet3 has… CONTINUE READING

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