Unique residues crucial for optimal editing in yeast cytoplasmic Leucyl-tRNA synthetase are revealed by using a novel knockout yeast strain.

@article{Yao2008UniqueRC,
  title={Unique residues crucial for optimal editing in yeast cytoplasmic Leucyl-tRNA synthetase are revealed by using a novel knockout yeast strain.},
  author={Peng Yao and Xiao-long Huang Liang Zhou and Ran He and M Xue and Yong-gang Zheng and Yue-fei Wang and En-Duo Wang},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 33},
  pages={22591-600}
}
Leucyl-tRNA synthetase (LeuRS) contains an editing domain that discriminates leucine from noncognate amino acids to ensure translational fidelity. In this study, a knock-out strain for Saccharomyces cerevisiae LeuRS was constructed to analyze in vivo the tRNA aminoacylation properties of S. cerevisiae and human cytoplasmic LeuRSs. The activities of several editing-defective mutants of ycLeuRS were determined in vitro and compared with those obtained in vivo in a complementation assay performed… CONTINUE READING