• Corpus ID: 26330990

Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN [correction of INF] and augment IFN-mediated [correction of INF] natural killer activity.

@article{Yamamoto1992UniquePS,
  title={Unique palindromic sequences in synthetic oligonucleotides are required to induce IFN [correction of INF] and augment IFN-mediated [correction of INF] natural killer activity.},
  author={S. Yamamoto and T. Yamamoto and Tetsuro Kataoka and Etsuro Kuramoto and Osamu Yano and Tohru Tokunaga},
  journal={Journal of immunology},
  year={1992},
  volume={148 12},
  pages={
          4072-6
        }
}
Thirty-mer single-stranded oligonucleotides, with a sequence chosen from the known cDNA encoding the 64-kDa protein named Ag A or the MPB-70 protein of Mycobacterium bovis BCG and the human cellular proteins such as complement component 1 inhibitor and Ig rearranged lambda-chain, were used to dissect the capability to induce IFN and to augment NK cell activity of mouse spleen cells by coincubation in vitro. Three with the hexamer palindromic sequence as GACGTC were active, whereas two kinds of… 
Hexamer palindromic oligonucleotides with 5'-CG-3' motif(s) induce production of interferon.
TLDR
The results strongly suggest that the minimal essential structure required for IFN induction is the hexamer palindromic sequence with CG motif(s), and that oligonucleotides with NACGTN and NTCGAN sequences exhibited the strongest activity.
Ability of oligonucleotides with certain palindromes to induce interferon production and augment natural killer cell activity is associated with their base length.
TLDR
It is indicated that the immunostimulatory activity of oligonucleotides with certain palindromic sequences requires an oligon nucleotide at least 18 bases long.
Binding of oligoguanylate to scavenger receptors is required for oligonucleotides to augment NK cell activity and induce IFN.
TLDR
It is suggested that the binding of an extrapalindromic sequence to the scavenger receptor is required for the immunostimulatory activity of oligo-1, a 30mer single-stranded oligonucleotide with oligoG sequences next to the active palindromo-G sequences.
Oligodeoxynucleotides containing palindrome sequences with internal 5'-CpG-3' act directly on human NK and activated T cells to induce IFN-gamma production in vitro.
TLDR
The results indicate that oligo-DNAs containing CG palindrome act directly on human NK cells and activated T cells to induce IFN-gamma production.
Divergent synthetic nucleotide motif recognition pattern: design and development of potent immunomodulatory oligodeoxyribonucleotide agents with distinct cytokine induction profiles.
TLDR
The ability for a divergent synthetic nucleotide motif recognition pattern of the receptor involved in the immunostimulatory pathway and the possibility of using synthetic nucleotides to elicit different cytokine response patterns are suggested.
The influence of DNA sequence on the immunostimulatory properties of plasmid DNA vectors.
TLDR
The results suggest that the addition of 20 to 60-base dG sequences to plasmids does not significantly affect their properties as immunostimulators or vaccines and suggest that MSR ligands can block cytokine induction by plasmid DNA whether or not theplasmid contains extended runs of dG.
Reactivity of human and porcine natural interferon-alpha producing cells to immunostimulatory DNA
TLDR
There are species differences in the recognition of immunostimulatory DNA and that eukaryotic DNA sometimes can be interferogenic, and the natural IFN-α producing cells (NIPC), also termed plasmacytoid dendritic cells (PDC), were the only cells among human or porcine PBMC that produced IFn-α in response to immunostIMulatory DNA.
Structural characterization of the inhibitory DNA motif for the type A (D)-CpG-induced cytokine secretion and NK-cell lytic activity in mouse spleen cells.
TLDR
A group of synthetic ODNs are designed, which were able to inhibit the induction of NK lytic activity, IL-12p40 and IFN-gamma cytokine secretion by type A (D)-CpG-ODNs, suggesting that CpG regulation of innate immunity can itself be regulated by particular motifs, which could be of therapeutic benefit in autoimmune diseases.
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