Unique interactions between the chromophore and glutamate 16 lead to far-red emission in a red fluorescent protein.

Abstract

mPlum is a far-red fluorescent protein with emission maximum at approximately 650 nm and was derived by directed evolution from DsRed. Two residues near the chromophore, Glu16 and Ile65, were previously revealed to be indispensable for the far-red emission. Ultrafast time-resolved fluorescence emission studies revealed a time dependent shift in the emission… (More)
DOI: 10.1002/pro.66

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