Unique conjugation mechanism in mycelial streptomycetes: a DNA-binding ATPase translocates unprocessed plasmid DNA at the hyphal tip.

@article{Reuther2006UniqueCM,
  title={Unique conjugation mechanism in mycelial streptomycetes: a DNA-binding ATPase translocates unprocessed plasmid DNA at the hyphal tip.},
  author={Jens Reuther and Cordula Gekeler and Yvonne Tiffert and Wolfgang Wohlleben and G{\"u}nther Muth},
  journal={Molecular microbiology},
  year={2006},
  volume={61 2},
  pages={
          436-46
        }
}
A single plasmid-encoded protein, the septal DNA translocator TraB, is sufficient to promote conjugal plasmid transfer in mycelial streptomycetes. To analyse the molecular mechanism of conjugation the closely related TraB proteins from plasmids pSG5 of Streptomyces ghanaensis and pSVH1 of Streptomyces venezuelae were characterized. TraB of pSG5 was expressed as a fusion protein with eGFP and found to be localized at the hyphal tips of Streptomyces lividans by fluorescence microscopy, which… CONTINUE READING

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