Unique and Conserved Features of Genome and Proteome of SARS-coronavirus, an Early Split-off From the Coronavirus Group 2 Lineage

@article{Snijder2003UniqueAC,
  title={Unique and Conserved Features of Genome and Proteome of SARS-coronavirus, an Early Split-off From the Coronavirus Group 2 Lineage},
  author={Eric J. Snijder and Peter J. Bredenbeek and Jessika C. Dobbe and Volker Thiel and John Ziebuhr and Leo L. M. Poon and Yi Guan and Mikhail Rozanov and Willy J. M. Spaan and Alexander E. Gorbalenya},
  journal={Journal of Molecular Biology},
  year={2003},
  volume={331},
  pages={991 - 1004}
}

Figures and Tables from this paper

Full-length genome sequences of two SARS-like coronaviruses in horseshoe bats and genetic variation analysis.

Genetic analysis demonstrated that a diverse G2b-CoV population exists in the bat habitat and has evolved from a common ancestor of SARS- coV.

Characterization of viral proteins encoded by the SARS-coronavirus genome

The ORF7b Protein of Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) Is Expressed in Virus-Infected Cells and Incorporated into SARS-CoV Particles

The ORF7b protein is not only an accessory protein but a structural component of the SARS-CoV virion, indicating that the sgRNA7 is bicistronic.

Characterisation of the transcriptome and proteome of SARS-CoV-2 reveals a cell passage induced in-frame deletion of the furin-like cleavage site from the spike glycoprotein

Background SARS-CoV-2 is a recently emerged respiratory pathogen that has significantly impacted global human health. We wanted to rapidly characterise the transcriptomic, proteomic and

Characterization of a Unique Group-Specific Protein (U122) of the Severe Acute Respiratory Syndrome Coronavirus

The deduced sequence contains a probable cleaved signal sequence and a C-terminal transmembrane helix, indicating that U122 is likely to be a type I membrane protein.

Characterisation of the transcriptome and proteome of SARS-CoV-2 using direct RNA sequencing and tandem mass spectrometry reveals evidence for a cell passage induced in-frame deletion in the spike glycoprotein that removes the furin-like cleavage site

Direct RNA sequencing using an Oxford Nanopore MinION characterised the transcriptome of SARS-CoV-2 grown in Vero E6 cells and detection of an apparently viable deletion in the furin cleavage site of the S glycoprotein reinforces the point that this and other regions of Sars-Co V-2 proteins may readily mutate.

Structure, Expression, and Intracellular Localization of the SARS-CoV Accessory Proteins 7a and 7b

The genome consists of several genes that are highly conserved and represent proteins with essential, basic functions in the viral life cycle, and an endoplasmic reticulum (ER) export signal residing in SARS-CoV is identified.

Mechanisms and enzymes involved in SARS coronavirus genome expression.

The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.

SARS-CoV ORF1b-encoded nonstructural proteins 12–16: Replicative enzymes as antiviral targets

Identification of Novel Subgenomic RNAs and Noncanonical Transcription Initiation Signals of Severe Acute Respiratory Syndrome Coronavirus

Evidence for derivation of the sgRNA core sequence from the body core sequence favor the model of discontinuous transcription during minus-strand synthesis, and one rare species of sg RNA has the junction sequence AAA, indicating that its transcription could result from a noncanonical transcription signal.
...

References

SHOWING 1-10 OF 84 REFERENCES

Mechanisms and enzymes involved in SARS coronavirus genome expression.

The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.

Coronavirus genome: prediction of putative functional domains in the non-structural polyprotein by comparative amino acid sequence analysis.

A partial tentative scheme for the functional organization and expression strategy of the non-structural polyproteins of IBV implies that, despite the general similarity to other positive strand RNA viruses, and particularly to potyviruses, coronaviruses possess a number of unique structural and functional features.

Comparison of genomic and predicted amino acid sequences of respiratory and enteric bovine coronaviruses isolated from the same animal with fatal shipping pneumonia.

The complete genome sequences are reported here of two field isolates of bovine coronavirus (BCoV), which were isolated from respiratory and intestinal samples of the same animal experiencing fatal

The carboxyl-terminal part of the putative Berne virus polymerase is expressed by ribosomal frameshifting and contains sequence motifs which indicate that toro- and coronaviruses are evolutionarily related.

Sequence analysis of the 3' part of the polymerase gene of the torovirus prototype Berne virus revealed that this area contains at least two open reading frames which overlap by 12 nucleotides, and homology with the ORF1b product of coronaviruses was revealed.

Coronaviruses

Genetic inactivation of exoN activity in engineered SArS-Cov and MHv genomes by alanine substitution at conserved De-D-D active site residues results in viable mutants that demonstrate 15to 20-fold increases in mutation rates, up to 18 times greater than those tolerated for fidelity mutants of other rNA viruses.

A comparative sequence analysis to revise the current taxonomy of the family Coronaviridae

Results of comparative sequence analysis of the spike (S), envelope (E), membrane (M), and nucleoprotein (N) structural proteins, and the two most conserved replicase domains, putative RNA-dependent RNA polymerase (RdRp) and RNA helicase (HEL), aimed at a revision of the Coronaviridae taxonomy are presented.

Equine arteritis virus is not a togavirus but belongs to the coronaviruslike superfamily

The data indicate that EAV, presently considered a togavirus, is evolutionarily related to viruses from the coronaviruslike superfamily.

The Genome Sequence of the SARS-Associated Coronavirus

The genome sequence reveals that the severe acute respiratory syndrome–associated coronavirus known as the Tor2 isolate is only moderately related to other known coronaviruses, andylogenetic analysis of the predicted viral proteins indicates that the virus does not closely resemble any of the three previously known groups of coronavirs.
...