Unique Self-Sustaining Circadian Oscillators Within the Brain of Drosophila melanogaster

  title={Unique Self-Sustaining Circadian Oscillators Within the Brain of Drosophila melanogaster},
  author={Shobi Veleri and Corinna W{\"u}lbeck},
  journal={Chronobiology International},
  pages={329 - 342}
In Drosophila circadian rhythms persist in constant darkness (DD). The small ventral Lateral Neurons (s-LNv) mainly control the behavioral circadian rhythm in consortium with the large ventral Lateral Neurons (l-LNv) and dorsal Lateral Neurons (LNd). It is believed that the molecular oscillations of clock genes are the source of this persistent behavior. Indeed the s-LNv, LNd, Dorsal Neurons (DN)-DN2 and DN3 displayed self-sustained molecular oscillations in DD both at RNA and protein levels… 
Organization of cell and tissue circadian pacemakers: A comparison among species
Real time, in vivo measurement of neuronal and peripheral clocks in Drosophila melanogaster
It is found that, while peripheral clocks in non-neuronal tissues were less stable after the loss of PDF signaling, they continued to oscillate, suggesting that the presumed dominance of the brain clock in regulating peripheral clocks needs to be re-examined.
The path to the 2017 Nobel Prize in Physiology or Medicine
Jeffrey C. Hall, Michael Rosbash and Michael W. Young discovered clock genes and their protein-products that control the circadian rhythm in the fruit fly, Drosophila melanogaster and their mutations that affect the fruitFly daily behaviours.
Light emission miracle in the sea and preeminent applications of bioluminescence in recent new biotechnology.
Bioluminescence imaging in living organisms.
Single live-cell imaging for systems biology.
Live-cell imaging and computational modelling are compatible techniques which allow quantitative analysis of cell signalling pathway dynamics, facilitating the identification of important qualitative and quantitative relationships-linking intracellular signalling, gene expression and cell fate.
The role of cryptochrome in the drosophila circadian clock
The role of CRYPTOCHROME in the Drosophila circadian clock is studied and it is shown that changes in the number of “good days” and “bad days�” in the cycle are regulated by different mechanisms.


Drosophila Free-Running Rhythms Require Intercellular Communication
It is shown that the brain circadian clock in Drosophila is clearly distinguishable from the eyes and other rapidly damping peripheral tissues, as it sustains robust molecular oscillations in DD and is the basis of sustained circadian locomotor activity rhythms.
Circadian rhythms in olfactory responses of Drosophila melanogaster
A robust circadian rhythm is demonstrated in Drosophila in electrophysiological responses to two classes of olfactory stimuli, providing evidence that peripheral circadian oscillators are necessary for circadian rhythms in Olfactory responses.
Defining the role of Drosophila lateral neurons in the control of circadian rhythms in motor activity and eclosion by targeted genetic ablation and PERIOD protein overexpression
In both LNv‐ablated and disco1 flies, PER cycles in the so‐called dorsal neurons (DNs) of the superior protocerebrum, suggesting that the weak short‐period rhythm could stem from these PDF‐negative cells.
Novel Features of Cryptochrome-Mediated Photoreception in the Brain Circadian Clock of Drosophila
It is shown here that cry is expressed in most, if not all, larval and adult neuronal groups expressing the PERIOD protein, with the notable exception of larval dorsal neurons (DN2s), and that dorsal PER-expressing brain neurons, the adult DN1s, are the only brain neurons to coexpress the CRY protein and the photoreceptor differentiation factor GLASS.
Clock mechanisms in Drosophila
Systems underlying circadian clock function in Drosophila melanogaster have been revealed by genetic and molecular approaches and light seems required for maintaining robust molecular oscillations at least in peripheral clock-gene-expressing tissues like the eyes, antennae, or Malpighian tubules.
The neuroarchitecture of the circadian clock in the brain of Drosophila melanogaster
The latest work on characterizing individual circadian pacemaker neurons in the fruit fly's brain that control activity and pupal eclosion is reviewed, leading to the questions by which neuronal pathways they are synchronized to the external light‐dark cycle, and how they impose periodicity on behavior.
Robust circadian rhythmicity of Drosophila melanogaster requires the presence of lateral neurons: a brain-behavioral study of disconnected mutants
It is shown that ventral lateral neurons (LNvs) are occasionally present and provoke robust circadian rhythmicity in disco mutants and it is suggested that the presence of single LNvs is sufficient to provoke robust rhythms in locomotor activity if the LNv terminals reach the superior protocerebrum.
Behavioral and molecular analyses suggest that circadian output is disrupted by disconnected mutants in D. melanogaster.
It is demonstrated that disco flies are rhythmic, indicating that they have an active circadian pacemaker that can be entrained by light and suggested that disco mutants block or interfere with elements of the circadian system located between the central pacemaker and its outputs that mediate overt rhythms.