Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis

@article{Jin2013UniqueOA,
  title={Unique Organizational and Functional Features of the Cytochrome c Maturation System in Shewanella oneidensis},
  author={M. Jin and Yaoming Jiang and Linlin Sun and Jianhua Yin and Huihui Fu and Gen-fu Wu and Haichun Gao},
  journal={PLoS ONE},
  year={2013},
  volume={8}
}
Shewanella are renowned for their ability to respire on a wide range of electron acceptors, which has been partially accredited to the presence of a large number of the c-type cytochromes. In the model species S. oneidensis MR-1, at least 41 genes encode c-type cytochromes that are predicted to be intact, thereby likely functional. Previously, in-frame deletion mutants for 36 of these genes were obtained and characterized. In this study, first we completed the construction of an entire set of c… Expand

Figures, Tables, and Topics from this paper

The Small RNA RyhB Is a Regulator of Cytochrome Expression in Shewanella oneidensis
TLDR
This study suggests that controlling cytochrome expression is of importance to maintain iron homeostasis and that sRNAs molecules are important players in the regulation of fundamental processes in S. oneidensis MR-1. Expand
Evidence for function overlapping of CymA and the cytochrome bc1 complex in the Shewanella oneidensis nitrate and nitrite respiration.
TLDR
A transposon random mutagenesis method in a cymA null mutant was adopted to identify substituent(s) of CymA with respect to nitrite and nitrate respiration, and confirmed that the cytochrome bc1 complex and CythA were indeed functionally overlapping in nitrate/nitrite respiration. Expand
Regulation and Maturation of the Shewanella oneidensis Sulfite Reductase SirA
TLDR
It is shown that maturation of SirA requires the combined function of the two apocytochrome c chaperones CcmI and SirG, and that CRP regulates expression of sirA via the newly identified transcriptional regulatory protein, SirR. Expand
Investigation of a spontaneous mutant reveals novel features of iron uptake in Shewanella oneidensis
TLDR
The results suggest that in S. oneidensis the siderophore-dependent pathway plays a key role in iron uptake when iron is limited, but many alternative routes exist, and lactate, but not other carbon sources that are routinely used to support growth, is able to promoteIron uptake when PutA is missing. Expand
Regulation of nitrite resistance of the cytochrome cbb3 oxidase by cytochrome c ScyA in Shewanella oneidensis
Cytochrome c proteins, as enzymes to exchange electrons with substrates or as pure electron carriers to shuttle electrons, play vital roles in bacterial respiration and photosynthesis. In ShewanellaExpand
Shewanella oneidensis cytochrome c maturation component CcmI is essential for heme attachment at the non‐canonical motif of nitrite reductase NrfA
TLDR
This study identifies SO0265 as CcmI, an apocytochrome c chaperone that is important and essential for maturation of c‐type cytochromes with the canonical heme binding motif(s) (HBM; CX2CH) and nitrite reductase NrfA carrying a non‐canonical C X2CK motif respectively. Expand
Evidence for the requirement of CydX in function but not assembly of the cytochrome bd oxidase in Shewanella oneidensis.
TLDR
CydX may function by positioning and stabilizing the prosthetic hemes, especially heme d in the CydA-CydB complex although a role of participating in catalytic reaction is not excluded, providing novel insights into the understanding of the small subunit of the cytochrome bd oxidase. Expand
Roles of multiple KASIII homologues of Shewanella oneidensis in initiation of fatty acid synthesis and in cerulenin resistance.
TLDR
It is demonstrated that FabH1 and temperature-responsive FabH2 are primarily responsible for initiating synthesis of straight- and branched-chain fatty acids respectively, whereas FabH3 and OleA are dispensable, implying that physiological function promiscuity of bacterial KAS enzymes could be more extensive than previously expected. Expand
Effects of FlrBC on flagellar biosynthesis of Shewanella oneidensis
TLDR
Evidence is presented to show that the difference in highly conserved polar flagellar systems can be surprisingly profound, even at the heart of the classical regulatory hierarchy. Expand
Positive regulation of the Shewanella oneidensis OmpS38, a major porin facilitating anaerobic respiration, by Crp and Fur
TLDR
It is shown that OmpS38 and OmpA are two major porins, neither of which is responsive to changes in osmolarity or contributes to the intrinsic resistance to β-lactam antibiotics. Expand
...
1
2
3
4
5
...

References

SHOWING 1-10 OF 47 REFERENCES
Genome sequence of the dissimilatory metal ion–reducing bacterium Shewanella oneidensis
TLDR
This genome sequence represents a critical step in the elucidation of the pathways for reduction (and bioremediation) of pollutants such as uranium (U) and chromium (Cr), and offers a starting point for defining this organism's complex electron transport systems and metal ion–reducing capabilities. Expand
Addressing Shewanella oneidensis "cytochromome": the first step towards high-throughput expression of cytochromes c.
TLDR
This work establishes a methodology for high-throughput expression of this class of proteins and provides a clone resource for the microbiological and functional genomics research communities. Expand
Identification of Mobile Elements and Pseudogenes in the Shewanella oneidensis MR-1 Genome
TLDR
A large number of mobile elements and multiple potential sites for integrase-mediated acquisition of foreign DNA indicate that the MR-1 genome is exceptionally dynamic, with many functions and regulatory control points in the process of decay or reinvention. Expand
Impacts of Shewanella oneidensis c‐type cytochromes on aerobic and anaerobic respiration
TLDR
It is suggested that an uncharacterized c‐type cytochrome (SO4047) may be important to both aerobiosis and anaerobiosis. Expand
Impact of the bacterial type I cytochrome c maturation system on different biological processes
TLDR
Novel hypotheses that should be explored include the utilization of Ccm‐associated haem for processes besides attachment to apocytochrome c, the export of a non‐haem compound through the Ccm system, and the negative effects of protoporphyrin IX accumulation. Expand
Genetic and Molecular Characterization of Flagellar Assembly in Shewanella oneidensis
TLDR
In conclusion, flagellar assembly in S. oneidensis has novel features despite the conservation of homologous genes across taxa. Expand
Identification of 42 possible cytochrome C genes in the Shewanella oneidensis genome and characterization of six soluble cytochromes.
TLDR
Results on regulation of cytochromes from DNA microarrays and 2D-PAGE differ somewhat from the results of Shewanella oneidensis MR-1, emphasizing the importance of multifaceted analyses in proteomics. Expand
Comparative c-type cytochrome expression analysis in Shewanella oneidensis strain MR-1 and Anaeromyxobacter dehalogenans strain 2CP-C grown with soluble and insoluble oxidized metal electron acceptors.
TLDR
Proteomic characterization proved to be a promising approach for determining the c-type cytochrome complement expressed under different growth conditions, and will help to elucidate the specific functions of more c- type cytochromes that are the basis for Shewanella and Anaeromyxobacter respiratory versatility. Expand
Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses
TLDR
The results indicate that the Arc system in S. oneidensis differs from that in E. coli substantially in terms of its physiological function and regulon while their binding motif are strikingly similar. Expand
Extracellular respiration of dimethyl sulfoxide by Shewanella oneidensis strain MR-1.
TLDR
Two gene clusters in Shewanella oneidensis strain MR-1 are identified that each contain homologs of genes required for metal reduction and genes that are predicted to encode dimethyl sulfoxide (DMSO) reductase subunits, suggesting these organisms may perceive DMSO in the environment as an insoluble compound. Expand
...
1
2
3
4
5
...