Unique GTP-binding pocket and allostery of uridylate kinase from a gram-negative phytopathogenic bacterium.

@article{Tu2009UniqueGP,
  title={Unique GTP-binding pocket and allostery of uridylate kinase from a gram-negative phytopathogenic bacterium.},
  author={Jhe-Le Tu and Ko-Hsin Chin and Andrew H-J Wang and S. B. Chou},
  journal={Journal of molecular biology},
  year={2009},
  volume={385 4},
  pages={
          1113-26
        }
}
Using X-ray diffraction methodology, we have successfully determined the tertiary structures of the apo- and GTP-bound forms of uridylate kinase (UMPK) from the gram-negative plant pathogen Xanthomonas campestris with crystals grown under a strong magnetic field. The flexible ATP- and UMP-binding loops are clearly shown under this situation. X. campestris UMPK contains a unique patch of noticeably positive nature from residue R100 to residue R127, allowing it to form a special GTP-binding… CONTINUE READING
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