Uninfected avian cells contain RNA related to the transforming gene of avian sarcoma viruses

  title={Uninfected avian cells contain RNA related to the transforming gene of avian sarcoma viruses},
  author={Deborah H. Spector and Karen Smith and Thomas Padgett and P A Mccombe and Daisy Roulland-Dussoix and Carlo Moscovici and Harold Varmus and J. M. Bishop},
Transforming Viruses in Cultures of Chicken
The transforming ability, the deleted genome, and the induced polyproteins of the SK viruses were reminiscent of the properties of several replication-defective acute transforming viruses.
DNA and RNA from Uninfected Vertebrate Cells Contain Nucleotide Sequences Related to the Putative Transforming Gene of Avian Myelocytomatosis Virus
The data suggest that evolution of oncogenic retroviruses has frequently involved a mechanism whereby incorporation and perhaps modification of different host genes provides each virus with the ability to induce its characteristic tumors.
Expression of cellular oncogenes.
Another category of c-one genes has been detected in the DNA of malignant cells by virtue of these genes’ ability to induce neoplastic transformation when “transfected” into a mouse fibroblastic cell line in vitro.
The transforming protein of avian sarcoma viruses and its homologue in normal cells.
  • R. Erikson
  • Biology
    Current topics in microbiology and immunology
  • 1981
Avian sarcoma viruses are a particularly useful and interesting group of agents for the study of biochemical aspects of malignant transformation and this fact may have contributed to a more rapid development of a molecular biologic description of these viruses than has been the case for other RNA tumor viruses.
Cellular Sequences Related to Three New onc Genes of Avian Sarcoma Virus (fps, yes, and ros) and Their Expression in Normal and Transformed Cells
It is demonstrated that UR2, the most recently isolated avian sarcoma virus, contains in its genome a unique sequence, ros, nonhomologous to src, fps, and yes sequences or to transforming genes of avian acute leukemia viruses.


DNA related to the transforming gene(s) of avian sarcoma viruses is present in normal avian DNA
It is demonstrated here that the DNA of normal chicken cells contains nucleotide sequences closely related to at least a portion of the transforming gene(s) of ASV; in addition, similar sequences are widely distributed among DNA of avian species and that they have diverged roughly according to phylogenetic distances among the species.
Avian Tumor Virus Proteins and RNA in Uninfected Chicken Embryo Cells
The content of proteins P19 and P15 of avian leukovirus in various types of uninfected chicken embryos has been determined by radioimmunoassay, suggesting that the genes for these proteins are coordinately controlled.
Detection of Avian Tumor Virus RNA in Uninfected Chicken Embryo Cells
Uninfected chicken embryo cells were analyzed for the presence of viral RNA by molecular hybridization with the single-stranded deoxyribonucleic acid (DNA) product of the RNA-dependent DNA polymerase contained in avian sarcoma-leukosis virions, and the viral RNA content correlated with the level of helper activity in the cells.
Nucleotide Sequence Relationships of Avian RNA Tumor Viruses: Measurement of the Deletion in a Transformation-Defective Mutant of Rous Sarcoma Virus
Evidence is provided that the majority, if not all, of the RNA sequences of Pr RSV-C deleted from its transformation-defective mutant are not represented in normal chicken DNA.
Temperature-Dependent Transformation of Cells Infected with a Mutant of Bryan Rous Sarcoma Virus
Inhibition of either protein or RNA synthesis, but not DNA synthesis, prevented the induction of increased hexose uptake and hyaluronic acid synthesis after a shift of RSV-BH-Ta-infected cells from 41 to 36 C, therefore, biochemical changes are secondary to a more basic change responsible for morphological transformation.
Requirement for Cell Division for Initiation of Transcription of Rous Sarcoma Virus RNA
Division of the RSV-infected chicken embryo fibroblast is required for the initiation of transcription of virus RNA, but it is not necessary for the maintenance of transcription in stationary cells.