• Biology, Medicine
  • Published in Journal of virology 1971

Unilateral synthesis of reovirus double-stranded ribonucleic acid by a cell-free replicase system.

@article{Sakuma1971UnilateralSO,
  title={Unilateral synthesis of reovirus double-stranded ribonucleic acid by a cell-free replicase system.},
  author={Sadashige Sakuma and Yoshiyuki Watanabe},
  journal={Journal of virology},
  year={1971},
  volume={8 2},
  pages={
          190-6
        }
}
A large-particle fraction obtained from reovirus-infected L cells contained both replicase and transcriptase activity. The in vitro replicase reaction slowed down soon after initiation, whereas the transcriptase reaction proceeded at an unabated rate. The replicase and transcriptase were both template-bound and could be separated from one another by controlled chymotryptic digestion followed by centrifugation in a CsCl gradient. The transcriptase was recovered as a sharp band (rho = 1.43) and… CONTINUE READING

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