Unfractionated human marrow cell cryopreservation using dimethylsulfoxide and hydroxyethyl starch.

@article{Stiff1983UnfractionatedHM,
  title={Unfractionated human marrow cell cryopreservation using dimethylsulfoxide and hydroxyethyl starch.},
  author={Patrick Stiff and Anthony J. Murgo and Charles G Zaroulis and M F DeRisi and Bayard D. Clarkson},
  journal={Cryobiology},
  year={1983},
  volume={20 1},
  pages={17-24}
}
Unfractionated bone marrow (BM) cells were cryopreserved in 1- to 2-ml aliquots using a mixture containing both 5% dimethylsulfoxide (DMSO) and 6% hydroxyethyl starch (HES) in an attempt to increase the viable cell yield and reduce the clumping after thawing, observed when 10% DMSO is used alone. Samples thawed after storage for 6 months in the vapor phase of liquid nitrogen, were assayed. Compared to prefreeze values, there was both a greater number of cells that excluded Trypan Blue (50… CONTINUE READING

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