Unexpected structural diversity in DNA recombination: the restriction endonuclease connection.

@article{Hickman2000UnexpectedSD,
  title={Unexpected structural diversity in DNA recombination: the restriction endonuclease connection.},
  author={Alison Burgess Hickman and Y Li and Shiny V. Mathew and E W May and Nancy L. Craig and Frederick Dyda},
  journal={Molecular cell},
  year={2000},
  volume={5 6},
  pages={1025-34}
}
Transposition requires a coordinated series of DNA breakage and joining reactions. The Tn7 transposase contains two proteins: TnsA, which carries out DNA breakage at the 5' ends of the transposon, and TnsB, which carries out breakage and joining at the 3' ends of the transposon. TnsB is a member of the retroviral integrase superfamily whose hallmark is a conserved DDE motif. We report here the structure of TnsA at 2.4 A resolution. Surprisingly, the TnsA fold is that of a type II restriction… CONTINUE READING