Undetectable histone O-GlcNAcylation in mammalian cells.


O-GlcNAcylation is a posttranslational modification catalyzed by the O-Linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and reversed by O-GlcNAcase (OGA). Numerous transcriptional regulators, including chromatin modifying enzymes, transcription factors, and co-factors, are targeted by O-GlcNAcylation, indicating that this modification is central for chromatin-associated processes. Recently, OGT-mediated O-GlcNAcylation was reported to be a novel histone modification, suggesting a potential role in directly coordinating chromatin structure and function. In contrast, using multiple biochemical approaches, we report here that histone O-GlcNAcylation is undetectable in mammalian cells. Conversely, O-GlcNAcylation of the transcription regulators Host Cell Factor-1 (HCF-1) and Ten-Eleven Translocation protein 2 (TET2) could be readily observed. Our study raises questions on the occurrence and abundance of O-GlcNAcylation as a histone modification in mammalian cells and reveals technical complications regarding the detection of genuine protein O-GlcNAcylation. Therefore, the identification of the specific contexts in which histone O-GlcNAcylation might occur is still to be established.

DOI: 10.1080/15592294.2015.1060387

Cite this paper

@article{Gagnon2015UndetectableHO, title={Undetectable histone O-GlcNAcylation in mammalian cells.}, author={Jessica Gagnon and Salima Daou and Natalia Zamorano and Nicholas V G Iannantuono and Ian Hammond-Martel and Nazar Mashtalir and {\'E}ric Bonneil and Hugo Wurtele and P. Thibault and El Bachir Affar}, journal={Epigenetics}, year={2015}, volume={10 8}, pages={677-91} }