Understanding the mechanism of virus removal by Q sepharose fast flow chromatography during the purification of CHO-cell derived biotherapeutics.

@article{Strauss2009UnderstandingTM,
  title={Understanding the mechanism of virus removal by Q sepharose fast flow chromatography during the purification of CHO-cell derived biotherapeutics.},
  author={Daniel M Strauss and Scott C Lute and Zinaida Tebaykina and Douglas D. Frey and Cintia Ho and Gregory S. Blank and Kurt A. Brorson and Qi Chen and Bin Yang},
  journal={Biotechnology and bioengineering},
  year={2009},
  volume={104 2},
  pages={371-80}
}
During production of therapeutic monoclonal antibodies (mAbs) in mammalian cell culture, it is important to ensure that viral impurities and potential viral contaminants will be removed during downstream purification. Anion exchange chromatography provides a high degree of virus removal from mAb feedstocks, but the mechanism by which this is achieved has not been characterized. In this work, we have investigated the binding of three viruses to Q sepharose fast flow (QSFF) resin to determine the… CONTINUE READING
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